1. Obtain 50mL sterile yellow top container and if patient <16 years of age also obtain a paediatric blood culture bottle. 2. Disinfect overlying skin with 2% iodine tincture or 70% alcohol. 3. Obtain specimen via percutaneous needle aspiration or surgery. 4. Split contents into the paediatric blood culture bottle if <16 years of age and into the sterile yellow top container. 5. Transport specimen to laboratory immediately.