Test Code: Mono Preferred Name Monospot test

30 minutes
As required
2 hours
Medicare Item

Test Tubes

ImageSpecimen TypeSpecimen VolumeTube Type
Serum (adult)8 mLSST with gel 8.5 mL (Dark Yellow Top)
Serum (paediatric)1 mL2x SST with gel 600 uL Paed (Red or Yellow top)


Test may be performed on serum, plasma or EDTA (whole blood).  Serum and plasma are the preferred specimens. Tube for the full blood count can be used for a monospot provided there is sufficient specimen.


APPLICATION:  The Monospot Test is a non-specific slide test for heterophil antibodies.  The results of the Monotest are often negative in infants and children under 4 years with proven EBV infection but it will identify approximately 90% of cases of EBV in older children and adults.

The test is simple and rapid and is performed daily in the Mater Laboratory.

Specifc serology for Epstein Barr Virus (EBV) is available and is referred to QHPS-RBH Laboratory for testing. Turn around times are of the order of several days.

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