Test Code: VitaD Preferred Name Vitamin D - 25 hydroxy

As requested
4 hours
Medicare Item with conditions - see General notes

Test Tubes

ImageSpecimen TypeSpecimen VolumeTube Type
Blood (adult)4 mLSST with gel 8.5 mL (Dark Yellow Top)
Blood (paediatric)1 mL2x SST with gel 600 uL Paed (Red or Yellow top)
Blood (neonatal)0.6 mLSST with gel 600 uL Paed (Red or Yellow top)


A number of Vitamin D assays are available. If in doubt as to which test is required, contact a Chemical Pathologist or Chemical Pathology Registrar.


In Item Requirement MUST be satisfied to be eligible for Medicare rebate - Item 66833 "For the investigation of a patient who:
(a) has signs or symptoms of osteoporosis or osteomalacia; or
(b) has increased alkaline phosphatase and otherwise normal liver function tests; or
(c) has hyperparathyroidism, hypo- or hypercalcaemia, or hypophosphataemia; or
(d) is suffering from malabsorption (for example, because the patient has cystic fibrosis, short bowel syndrome, inflammatory bowel disease or untreated coeliac disease, or has had bariatric surgery); or
(e) has deeply pigmented skin, or chronic and severe lack of sun exposure for cultural, medical, occupational or residential reasons; or
(f) is taking medication known to decrease 25OH-D levels (for example, anticonvulsants); or
(g) has chronic renal failure or is a renal transplant recipient; or
(h) is less than 16 years of age and has signs or symptoms of rickets; or
(i) is an infant whose mother has established vitamin D deficiency; or
(j) is a exclusively breastfed baby and has at least one other risk factor mentioned in a paragraph in this item; or
(k) has a sibling who is less than 16 years of age and has vitamin D deficiency.

Such information must be included in the clinical notes on the request form.  

For advice on Vitamin D testing, contact a Chemical Pathologist.

APPLICATION:  Investigation of suspected vitamin D deficiency or toxicity.

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