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Test Code: TB Preferred Name Blood culture Mycobacteria

No
< 6 weeks
Daily
< 6 weeks
Medicare Item
N/A

Test Tubes

ImageSpecimen TypeSpecimen VolumeTube Type
Blood8 - 10 mLBacT/Alert Myco MB, 3-5 mL (Black cap Mycobacteria)

COLLECTION NOTES

DISINFECTION  CULTURE BOTTLE:
Apply 70% isopropyl alcohol to the rubber stopper and wait 1 minute.

DISINFECTION OF VENIPUNCTURE SITE:
1. Cleanse site with Betadine (except neonates, allow to dry and then wipe with 70% alcohol (Alcowipe).
2. Starting at the centre, swab concentrically with the iodine preparation.

COLLECTION OF BLOOD:
1. Aseptically collect 8-10 mL of blood (1-3 mL for children).  Larger volumes increase the sensitivity.
2. Inject blood into TB Bottle.
3. Do not collect through catheter or cannula.
4. Do not refrigerate.  Send to the laboratory.

GENERAL NOTES



1. TB bottle contains antibiotics which may inhibit non-mycobacterial organisms.
2. Highly immunosuppressed patients may be at risk for Mycobacterial bacteraemia.

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